RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros Beetle Trypoxylus dichotomus

RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros Beetle Trypoxylus dichotomus

Blog Article

In the Japanese rhinoceros beetle Trypoxylus dichotomus, various candidate genes required for a specific phenotype of interest are listed by next-generation sequencing analysis.Their functions were investigated using Horse Pilot XDesign Breeches Ladies RNA interference (RNAi) method, the only gene function analysis tool for T.dichotomus developed so far.The summarized procedure for the RNAi method used for T.dichotomus is to synthesize double-stranded RNA (dsRNA), and inject it in larvae or pupae of T.

dichotomus.Although some dedicated materials or equipment are generally Horse Cooler Rugs required to inject dsRNA in insects, the advantage of the protocol described here is that it is possible to inject dsRNA in T.dichotomus with one syringe.